Coding Phases / Frames
The phase (or sometimes called frame) gives information on how to translate individual parts of a gene, the coding exons. Phases 1 & 2 have a different definition in GFF and EnsEMBL format!
Category: genome informatics
Determining Coverage for NGS Data
One of the most common question after your Next-Generation Sequencing (NGS) run is: “What is the coverage I achieved?” Unfortunately there a different definitions available for the term “coverage”.
Create BigWig files with DeepTools
To display data as bar graphs along the genome e.g. in the UCSC genome browser, you can create BigWig files. DeepTools is a powerful library that can also help with this.