There are different ways to encode the quality scores in FASTQ files from Next-generation sequencing machines. This is important to find out before using the data and to convert between formats if necessary.
One of the most common question after your Next-Generation Sequencing (NGS) run is: “What is the coverage I achieved?” Unfortunately there a different definitions available for the term “coverage”.
Notes about working with fasta / fastq files on the Unix command line.
Some of the landmarks in the history of DNA sequencing and molecular biology.
Notes about blc files, written as part of the primary analysis of Illumina sequencing machines.