This is an introductory article about Pre-Implantation Genetic Testing for Aneuploidy (PGT-A, formerly known as PGS) using sequencing-based read-counting. The procedure is simplified and I am avoiding to talk about specific companies or products here. Increasing the chances of a successful pregnancy by looking at the embryos chromosomes During an IVF cycle there are – in […]
There are different ways to encode the quality scores in FASTQ files from Next-generation sequencing machines. This is important to find out before using the data and to convert between formats if necessary.
One of the most common question after your Next-Generation Sequencing (NGS) run is: “What is the coverage I achieved?” Unfortunately there a different definitions available for the term “coverage”.
Notes about working with fasta / fastq files on the Unix command line.
Some of the landmarks in the history of DNA sequencing and molecular biology.