RNA-Seq data quality scores
There are different ways to encode the quality scores in FASTQ files from Next-generation sequencing machines. This is important to find out before using the data and to convert between formats if necessary.
Category: sequencing
Determining Coverage for NGS Data
One of the most common question after your Next-Generation Sequencing (NGS) run is: “What is the coverage I achieved?” Unfortunately there a different definitions available for the term “coverage”.
Command-line NGS data munging
Notes about working with fasta / fastq files on the Unix command line.
A DNA Sequencing History
Some of the landmarks in the history of DNA sequencing and molecular biology.
BCL files
Notes about blc files, written as part of the primary analysis of Illumina sequencing machines.