Category: bioinformatics
Determining Coverage for NGS Data
One of the most common question after your Next-Generation Sequencing (NGS) run is: "What is the coverage I achieved?" Unfortunately there a different definitions available for the term "coverage".
Command-line NGS data munging
Notes about working with fasta / fastq files on the Unix command line.
Software Requirements Specification
For any large software project (i.e. one that requires more than a few scripts performing a one-off task) and for every project that was initiated by a customer request, it…
Sequence Mappability & Alignability
Sequence uniqueness within the genome plays an important part when attempting to map short sequence parts – e.g. next-generation short sequencing reads. It is one of the factors that can…