Determining Coverage for NGS Data

One of the most common question after your Next-Generation Sequencing (NGS) run is: "What is the coverage I achieved?" Unfortunately there a different definitions available for the term "coverage".

Command-line NGS data munging

Notes about working with fasta / fastq files on the Unix command line.

Software Requirements Specification

For any large software project (i.e. one that requires more than a few scripts performing a one-off task) and for every project that was initiated by a customer request, it…

BCL files

Notes about blc files, written as part of the primary analysis of Illumina sequencing machines.

Sequence Mappability & Alignability

Sequence uniqueness within the genome plays an important part when attempting to map short sequence parts – e.g. next-generation short sequencing reads. It is one of the factors that can…