CRAM format notes

CRAM files are compressed versions of BAM files containing (aligned) sequencing reads. They represent a further file size reduction for this type of data that is generated at ever increasing…

RNA-Seq data quality scores

There are different ways to encode the¬†quality scores in FASTQ files¬†from Next-generation sequencing machines. This is important to find out before using the data and to convert between formats if…

Determining Coverage for NGS Data

One of the most common question after your Next-Generation Sequencing (NGS) run is: "What is the coverage I achieved?" Unfortunately there a different definitions available for the term "coverage".