CRAM format notes
CRAM files are compressed versions of BAM files containing (aligned) sequencing reads. They represent a further file size reduction for this type […]
Tag: NGS
RNA-Seq data quality scores
There are different ways to encode the quality scores in FASTQ files from Next-generation sequencing machines. This is important to find out before using the data and to convert between formats if necessary.
Determining Coverage for NGS Data
One of the most common question after your Next-Generation Sequencing (NGS) run is: “What is the coverage I achieved?” Unfortunately there a different definitions available for the term “coverage”.