CRAM format notes

CRAM files are compressed versions of BAM files containing (aligned) sequencing reads. They represent a…

RNA-Seq data quality scores

There are different ways to encode the¬†quality scores in FASTQ files¬†from Next-generation sequencing machines. This…

Determining Coverage for NGS Data

One of the most common question after your Next-Generation Sequencing (NGS) run is: "What is…